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polyacrylamide precursor solution  (Thermo Fisher)


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    Thermo Fisher polyacrylamide precursor solution
    Polyacrylamide Precursor Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyacrylamide+precursor+solution/Acrylamide+(30%25)+Solutions/pmc11981600-53-1-10
    Average 94 stars, based on 1 article reviews
    polyacrylamide precursor solution - by Bioz Stars, 2026-08
    94/100 stars

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    (A) Immortalized monocytes were primed to macrophages by adding 30ng/mL PMA. The macrophages were then polarized in three ways: M0 (naïve) without PMA, M1 (proinflammatory) with 10ng/mL LPS, 5ng/mL IFN- γ , and 30ng/mL PMA, and M2 (anti-inflammatory) with 25ng/mL IL-4, 25ng/mL IL-13, 30ng/mL PMA. (B) Macrophages directly activated on collagen type 1 (Col1)-coated soft or stiff polyacrylamide (PA) gels. After macrophage activation, human mammary epithelial cells (MCF10A) were seeded into a monolayer atop the macrophages.

    Journal: bioRxiv

    Article Title: Epithelial multicellular clustering enabled by polarized macrophages on soft matrices

    doi: 10.1101/2023.02.20.529258

    Figure Lengend Snippet: (A) Immortalized monocytes were primed to macrophages by adding 30ng/mL PMA. The macrophages were then polarized in three ways: M0 (naïve) without PMA, M1 (proinflammatory) with 10ng/mL LPS, 5ng/mL IFN- γ , and 30ng/mL PMA, and M2 (anti-inflammatory) with 25ng/mL IL-4, 25ng/mL IL-13, 30ng/mL PMA. (B) Macrophages directly activated on collagen type 1 (Col1)-coated soft or stiff polyacrylamide (PA) gels. After macrophage activation, human mammary epithelial cells (MCF10A) were seeded into a monolayer atop the macrophages.

    Article Snippet: Polyacrylamide precursor solutions were mixed by adjusting 40% acrylamide (Bio-Rad: 161-0142) and 2% bis-acrylamide (Bio-Rad: 161-0140).

    Techniques: Activation Assay